Molecular Cloning

Molecular cloning is an essential technique for creating DNA-based experimental tools for expression in bacterial or mammalian cells. Examples of such DNA constructs include promoter elements fused to reporter genes or cDNA sequences under the control of ubiquitous promoters. Molecular cloning includes preparation of vector and insert DNA, ligation of inserts into the vector, transformation of competent E. coli, and identification of positive clones.

Molecular cloning creates multiple molecules. Cloning is often used to amplify fragments of DNA that contain the entire gene, but it can also be used to amplify any DNA sequence, such as a promoter or non-coding sequence or randomly fragmented DNA. It is used in a variety of biological experiments and practical applications, from gene fingerprinting to large-scale protein production. The term cloning is sometimes misleadingly used to refer to the identification of chromosomal positions in genes associated with a particular phenotype of interest, such as location cloning. In practice, the location of a gene on a chromosome or genomic region does not necessarily allow the isolation or amplification of related genomic sequences. In order to amplify an organism's DNA sequence, it must be linked to the origin of replication. An origin of replication is a DNA sequence that can direct the replication of a sequence linked to itself However, many other functions are required, protein production, affinity labeling, single-strand RNA or DNA production, and hosts enable other molecular biology tools.

Cloning a DNA fragment basically involves four steps

  • screening/selection – selecting out the cells that were successfully transfected with the new DNA

Related Associations and Societies

Australasian Microbiology SocietyBrazilian Society of MicrobiologyGerman Society of Hygiene and MicrobiologyItalian Society for MicrobiologySpanish Society for Microbiology, Swedish Society for Microbiology.


    Related Conference of Molecular Cloning

    May 04-05, 2023

    52nd World Congress on Microbiology

    London, UK
    May 15-16, 2023

    27th World Congress on Biotechnology

    Prague, Czech Republic
    July 03-04, 2023

    15th International Virology Summit

    London, UK
    July 10-11, 2023

    8th International Conference on Bacteriology

    Valencia, Spain
    July 26-27, 2023

    29th Global Summit on Bioinformatics

    Montreal, Canada
    October 25-26, 2023

    10th World Congress on Microbiology & Infectious Diseases

    Zurich, Switzerland
    November 13-14, 2023

    11th Global Medical Microbiology Summit & Expo

    Edinburgh, Scotland
    November 15-16, 2023

    11th World Congress and Expo on Applied Microbiology

    Barcelona, Spain

    Molecular Cloning Conference Speakers

      Recommended Sessions

      Related Journals

      Are you interested in