M. Zamri-Saad
University Putra Malaysia, Malaysia
Title: Phagocytosis and Killing of Brucella melitensis by Phagocytic Cells and Lymphocytes of Goats
Biography
Biography: M. Zamri-Saad
Abstract
Phagocytosis and Killing of Brucella melitensis by Phagocytic Cells and Lymphocytes of Goats: This paper describes the phagocytosis and killing efficiency of the phagocytic cells, and lymphocytes of goats. Six adult male goats were divided into 2 groups. Group 1 was exposed subcutaneously to 109 cfu/mL of killed B. melitensis while Group 2 was similarly exposed to sterile PBS. After 2 weeks, blood samples were collected. The neutrophils, monocytes and lymphocytes were isolated and prepared as monolayer. Then, 200 µL of an inoculum containing 107 cfu/mL of live
B. melitensis was introduced and incubated at 370C in 5% CO2. At 0, 30, 60 and 120 min of incubation, they were harvested, stained with acridine orange and observed under fluorescence microscope at x1000 magnification. A minimum of 100 cells were examined for the presence and viability of bacteria.
Phagocytosis by neutrophils and macrophages showed increasing pattern with time. Earlier exposure significantly (p<0.05) enhanced the phagocytic activity after 60 min and 120 min by neutrophils and macrophages, respectively. Penetration into the lymphocytes also increased with time and earlier exposure significantly (p<0.05) enhanced the penetration after 60min. There was insignificant (p>0.05) killing efficiency by the neutrophils but the macrophages showed significant (p<0.05) increasing pattern after 60min. Similarly, survival of B. melitensis within the lymphocytes showed insignificant (p>0.05) decreasing pattern. In conclusion, exposure to killed B. melitensis significantly (p<0.05) enhanced the phagocytosis and killing efficiency of macrophages but insignificantly (p>0.05) improved the killing by neutrophils and lymphocytes.