Muhammad Atique Arshad
Government College University Faisalabad, Pakistan
Title: Auxin Biosynthesis Potential of different Plant Growth Promoting Rhizobacteria in the presence of Precursor L-Tryptophan
Biography
Biography: Muhammad Atique Arshad
Abstract
The Auxin biosynthesis is crucial to plant growth and development. Indole-3-Acetic Acid is the naturally occurring and the most studied Auxin in plants. It is a major Phyto hormone that controls and coordinates plant development. The present study was conducted to illustrate the pronounced potential of different Plant Growth Promoting Rhizobacteria (PGPR’s) in Auxin Biosynthesis in the presence of Growth precursor L-Tryptophan. Tryptophan is a non-polar, aromatic-amino acid used as a Building block in Protein biosynthesis. PGPR’s are a diverse group of bacteria inhabiting plant roots and are involved in modulating plant growth and development by a number of mechanisms including Auxin biosynthesis which is immensely considerable. This study involved the isolation and culturing of purified isolates of Eight Different PGPR’s to which Maize seeds were soaked. Different concentrations of L-Tryptophan were then added and these seeds were sown. Appropriate environmental conditions and proper fertilization was given. The mature plants were then subjected to analyze their Auxin level by using Spectrophotometer. Two PGPR’s i.e. Azotobacter and Azospirillum showed pronounced Auxin level (5.983ug/ml and 5.034ug/ml of IAA Equivalents respectively). It is therefore concluded that the maize plants showed tremendous Auxin Biosynthesis when they were subjected to growth precursor L-Tryptophan and PGPR’s which govern this synthesis. Such PGPR’s may be used to explore the biosynthesis potential of other phyto-hormones using different amino acids as precursors in future.