Abdulrazak M. H
Sule Lamido University , Nigeria
Title: Anti-Salmonella, Cytotoxicity and GC-MS Analysis of Leaves Extracts of Carica papaya
Biography
Biography: Abdulrazak M. H
Abstract
Shed dried matured leaves of Carica papaya were extracted with water, ethanol and chloroform solvents via percolation method. The composition of bioactive compounds in the leaves extracts of Carica papaya was determined by Gas Chromatography Mass Spectrophotometry. The toxicity statuses of the extracts were tested using Brine Shrimp Lethality Assay and bioactivity test was carried out on the bioactive TLC fractions. From the GC-MS study eighteen compounds were identified, with oleic acid (36.65%), palmitic acid (16.68%), stearic acid (9.78%) and 9-octadecenal (7.44%) being the major components. The toxicity study revealed that chloroform extracts of C. papaya was not toxic with LC50 value of (11,641µg/ml). While water extracts have LC50 values of (57.450µg/mL) and ethanolic extracts presents LC50 values of (179.505 µg/mL) respectively. Lastly results of antibacterial activity using contact bio-autography showed that the only bioactive fractions (Rf2 and Rf3 of chloroform extracts) were active against Salmonella Typhi and Salmonella Paratyphi B only.