Patamabhorn Amavisit
Kasetsart University, Thailand
Title: Plasmid-mediated antimicrobial resistances of V. parahaemolyticus from Pacific white shrimp and E. coli from diarrheal swine.
Biography
Biography: Patamabhorn Amavisit
Abstract
Vibrio parahaemolyticus isolated from the Pacific white shrimp (Litopenaeus vannamei) and Escherichia coli isolated from intestinal organs of diarrhea swine were tested antimicrobial sensitivity and studied on plasmid-mediated antimicrobial resistance genes. The antimicrobial resistant (AMR) rates of V. parahaemolyticus were 98.48% ampicillin, 3.03% doxycycline, 4.55% oxytetracycline, 6.06% erythromycin, 1.52% florfenicol, and 1.52% sulphamethoxazole-trimetroprime. V. parahaemolyticus were not resistant to tested quinolone agents (ciprofloxacin, enrofloxacin, norfloxacin and ofloxacin), but some isolates presented intermediate susceptibility of the agents. Plasmid-mediated quinolone resistance (PMQR) gene, qnrVC was found in only one isolate. DNA amplification of pirAB-like genes, which caused acute hepatopancreatic necrosis disease (AHPND) in shrimp, showed that 39.39% of V. parahaemolyticus carried this virulent gene. However acquisition of pirAB-like gene virulent factor in V. parahaemolyticus was not related to their AMR. While the AMR rates of E. coli were relatively high at 98.41% amoxicillin, 98.41% ampicillin, 96.83% cephalexin, 69.84% ciprofloxacin, 69.84% enrofloxacin, 57.14% fosfomycin, 77.78% colistin, 84.13% gentamicin, and 90.48% oxytetracycline. The rates of E. coli carried PMQR genes were 61% of qnrS and 9.5% of oqxA. Colistin resistant gene mcr-1 that located on plasmid was also amplified and found that 25.40% of the isolates carried mcr-1 gene and they had colistin MIC  2 μg/mL.