Obiekezie S.O
Department of Microbiology, Nigeria State
Title: Multidrug resistance of escherichia coli islolated from retailed smoked and iced fish in Keffi. Nasawara state, Nigeria
Biography
Biography: Obiekezie S.O
Abstract
Multidrug resistance of Escherichia coli isolated from retiled and iced fish sold in Keffi metropolis, Nigeria was carried out. A total number of thirty two (32) samples (16 smoked and 16 iced fish) from different location such as Angwan Lambu, High Court, Dadin Kowa and Market were collected. The isolation and identification of E. coli isolated were carried out using a method described by Clinical Laboratory Standard Institute (CLSI). Out of 32 samples of the smoked and iced fish collected, the percentage occurrence of E. coli was 100%. The total bacteria cunt (x106cfu/ml) ranged from 8.2-9.8, 4.6-7.8, 3.2-8.2 and 3.2-9.2 in Angwan Lambu, High Court, Dadin Kowa and Market respectively. Total coliform count (x106cfu/ml) ranged from 1.0-16, 1.6-4.2, 7.2 - 2.0 and 7.9-2.1 in Angwan Lambu, High Court, Dadin Kowa and Market respectively for smoked fish. Also, the total bacteria count (x106cfu/ml) ranged from 3.0-6.0, 4.3-6.1, 3.6-4.8 and 4.7, and 4.3-12.3 in Angwan Lambu, High Court, Dadin Kowa and Market respectively. Total faecal count (x106cfu/ml) ranged from 1.0-2.0, 1.4-2.0, 1.2-2.8, and 1.8-4.3 in Angwan Lambu, High Court, Dadin Kowa and Market respectively. The percent resistance pattern of E. coli isolates to antibiotics tested where Chloramphenicol (56.3%), Septrin and Sparfloxacin (46.9%), Augmentin (43.6%), Ampicillin (37.5%), Ciprofloxacin (34.4%), Perfloxacin (31.3%), Oflaxacin and Streptomycin (25%) and Gentamicin (18.6%). All the E. coli were multidrug resistant with multiple antibiotic resistance (MAR) isolates with (35.3) being jointly resistant to six antibiotics. MAR indices were also high (all above 0.2) prior exposure of the E. coli isolates to antibiotics.