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Hisham N Altayb

Hisham N Altayb

Sudan University of Science and Technology, Sudan

Title: Molecular Characterization of CTX-M ESBLs among Pathogenic Enterobacteriaceae isolated from different regions in Sudan

Biography

Biography: Hisham N Altayb

Abstract

This is across sectional study conducted to detect and characterize CTX-M genes among extended spectrum β-lactamases (ESBLs) producing Enterobacteriaceae isolated from different regions in Sudan. A total of 305 of clinical Enterobacteriaceae spp. Isolates, were collected from different regions in Sudan. ESBLs production was initially screened by cefotaxime, cefepime and ceftazidime, and then confirmed by disk combination method and PCR. DNA sequencing was done to differentiate between, blaCTX-M genotypes. Escherichia coli, was the most predominant (58%) isolate, followed by Klebsiella pneumoniae (26.6%), Citrobacter freundii (3.6%), Enterobacter species (6.2%) and Proteus species (5.6%). ESBLs were detected by disk combination method in 128(42%) isolates; Khartoum state 64% (23/36), Gizera state 54% (54/100), Sinnar state 53% (49/92) and White Nile state 2.6% (2/77).Three quarters of the ESBLs producers (96/128), were positive for blaCTX-M genes by PCR. blaCTX-M-15, was the most reported one 78.3% (18/23), followed by blaCTX-M-1413% (3/23), blaCTX-M-27 4.3% (1/23) and blaCTX-M-98 4.3% (1/23).There was a transition mutation (substitution of A with G at position 25) in the blaCTX-M gene (ID: KP309815), that affect protein structure.We concluded that,blaCTX-M-15 was the most commonly encountered genes and widely spread in different Sudanese regions.