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Hafid Soualhine

Hafid Soualhine

National Public Health Institute of Quebec, Canada

Title: Spread of M. chimaera in cardiac surgery operating room in quebec: WGS and RTPCR for outbreak investigation and management

Biography

Biography: Hafid Soualhine

Abstract

Mycobacterium chimaera outbreaks affecting patients subjected to open-chest heart surgery have been reported worldwide and associated with a high case fatality rate, reinforcing the need for strongly coordinated surveillance and outbreak control. The ECDC, Health Canada, the U.S. Centers for Disease Control and Prevention (CDC) and others issued warnings regarding the
potential risk of infection with M. chimaera for patients underwent open-heart surgery since 2012. The public health reference laboratory in Quebec undertook a laboratory active surveillance screening of all sterile site samples collected from patients presenting with M. chimaera -positive results between 2010 and 2016. The surveillance of environmental and clinical cases was extended to
the nine busiest cardiac surgery centers in Quebec. In this study, we attempted to determine the source of M. chimaera infection by establishing a molecular link between patients (n=7) and environmental strains (n=32). We developed a genome-wide strain genotyping approach and investigated the relatedness of M. chimaera isolates from patients and heat-cooler units (HCUs) from different geographical locations in Quebec. Four clinical isolates were associated to the European outbreak. We also implemented a new rapid molecular test based on quantitative real-time PCR targeting the ITS regions. This allowed us to detect rapidly the presence of M. chimaera in water samples and compare results with culture, follow up the bacterial burden in 26 contaminated HCUs. In conclusion, four patients infected with M. chimaera during cardiac surgery were related to one site in Montreal and were associated to the worldwide outbreak. Moreover RT-PCR allowed us to monitor and follow up the contamination in HCU in operating rooms.