8^th Clinical Microbiology Conference

Background & Aim: H. pylori were found to be an important factor in the pathogenesis of peptic ulcer disease. The growing problem of antibiotic resistance by the organism in Pakistan thus demands the search for novel compounds; we screened the aqueous extracts of Camellia sinensis and also compared the anti-bacterial

Materials & Methods: All these biopsies were processed for detection of H. pylori by two rapid helicourease – indigenously developed rapid urease detection kits, culture and polymerase chain reaction (PCR).The 5% aqueous extract of fermented, semi fermented and non-fermented Green tea was prepared and their antibacterial potential was explored against 35 clinical isolates of H. pylori agar well diffusion technique. The Minimum Inhibitory Concentration (MIC) of the most susceptible tea products were also carried out by Micro-dilution method for the sake of comparison.

Results: Most of the screened H. pylori isolates were resistant to more than one of the antibiotics like metronidazole and clarithromycin. The most significant activity was obtained in non-fermented green tea with an average zone of inhibition along with MIC was around 32 mm (MIC 120-150 á»±g/ml), semi fermented product showed 28 mm (MIC 140-200 á»±g/ml) showed and fermented one showed 32 mm (MIC 120-200 á»±g/ml).

Conclusion: In conclusion, our results indicated that all processed forms of green tea extracts possessed some variable level of anti H. pylori activity. But non-fermented that is freshly plucked green tea leaves without any industrial treatment has profound effect with least MIC and thus considered as a suitable and safe candidate for the eradication of H. pylori particularly drug resistant species.