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Latre Buntaran

Latre Buntaran

Mayapada Hospitals,Indonesia

Title: Community-Acquired Methicillin-Resistant Staphylococcus aureus: characteristics epidemiology, clinical, microbiology and molecular biology ; SCCmec gene analysis and VISA in patients infected with Staphylococcus aureus and antibiotic resistance testing in 2 hospitals

Biography

Biography: Latre Buntaran

Abstract

Community Acquired Methicillin Resistant Staphylococcus aureus (CA-MRSA) is a strain of MRSA that can cause infections in patients in the community, in which these patients had no previous risk factors for MRSA infection and the patient received 72 hours prior to infection when admitted to hospital.This study aims to determine and compare the characteristics of epidemiological, clinical, and molecular biology of CA-MRSA with HA-MRSA.

           Of the 311 S.aureus isolates collected from 2 hospitals (RSAB Harapan Kita and RS Siloam Kebun Jeruk) during the period 2009 to 2011, the prevalence of MRSA is 6% and consists of CA-MRSA (2%) and HA-MRSA (4%) , the pattern of infection as follows : SSTI (skin and soft tissue infections) : 56%, UTI (urinary tract infection) : 17%, RSA (acute rhino sinusitis) : 11%, Pneumonia : 6%, febrile observation : 5% and ILO (wound infection) : 5%. The third-generation cephalosporins and quinolones are the antibiotics most used in this study. These third-generation cephalosporins are resistant to all isolates of MRSA (CA-MRSA and HA-MRSA), whereas quinolone resistant to HA-MRSA, but still sensitive to CA-MRSA. The use of antibiotics against infections by S.aureus of 311 isolates showed that the use of antibiotics : inappropriate : 57%,  appropriate : 43%, adequate : 43%, inadequate : 57%, oral : 79%, parenteral : 21%, original : 36% and copy product : 64%.                

          Futhermore, 11 strains of Staphylococcus aureus were performed by PCR,  in which, there is one strain of Community-Acquired MRSA (CA-MRSA) with SCCmec type II, 3 strains of Hospital-Acquired MRSA (HA-MRSA) with SCCmec type IV, and two strains of Hospital-Acquired MSSA (HA-MSSA) and five strains of Community Acquired MSSA (CA-MSSA) that do not contain mecA genes and SCCmec. From the three strains of one strain of CA-MRSA and two strains HA-MRSA containing plasmid pUB110. vraA present in 91% of the 11 strains,  vraF : 36% , vraG : 45%, and vraR : 36%. Noteworthy, strains without pUB110 contained in a relatively high frequency of 75% in vraR as well as vraF, and 70% in vraA compared to strains with pUB110 : 60% in vraG.