6th Clinical Microbiology Conference

Biography

Biography: Demet Çelebi

Abstract

Aim: The increase in antibiotic-resistant bacteria has dramatically revived the interest in plant products as alternative anti microbial agents to prevent the efficiency of pathogenic microorganisms. Our aim in this study is to show the anti microbial activities of cinnamon (Cinnamomum cassia), tarragon (Artemisiadra cunculus) and ginger (Zingiber officinale) oils against the clinically important bacteria and yeasts. Materials and method: The anti microbial activity of the cinnamon, tarragon and ginger oils were tested against Citrobacter spp., Klebsiella spp., Proteusmirabilis, Pseudomonas, MRKNS, Enterococcusspp., Staffilococcusaureus, Enterobacteraerugenes, Candida spp and E.coli. Essential oils of cinnamon,tarragon and ginger were extracted by steam distillation, final yield of extraction 0.5, the volume of EO extract was adjusted to 100 ml with sterile distilled water, thus obtaining the crude essential oil extracts used for anti microbial tests. Disc diffusion method (Kirby-Bauer) was used to show the anti microbial activity by measuring the zone diameters. Turbidity was visually adjusted to that of a 0.5 McFarland turbidity standard (1.5x 10 ~ CFU/ml) using sterile Mueiler-Hinton broth and after 24 hours. 0.1 ml for each oils, Sterile filter paper disks were prepared to a diameter of 6.35 mm and sterilized in a Pasteur-oven, (at 170~ for 2 hour). Volatile oils and extracts were sterilized by passing through 0.22 mm pore-size membrane filters and then 0.02 ml of the solution of volatile oils was pipetted into the center of each disk to achieve the desired potency. Disks were air-dried in a contamination free environment. Results:The cinnamon oil show an anti microbial activity against the tested micro organisms with different zone. Citrobacter 25mm, Klebsiella 18mm, P.mirabilis 23 mm, Pseudomonas 15mm, MRKNS 22mm, Enterococcus 70 mm, S.aureus 32 mm, Enterobacter 19 mm, Candidaspp 18 mm and E.coli 10 mm. Ginger and tarrogon oil wasn’t show an anti mirobial activity against bacteria and candida. Conclusion:.The cinnemon oil has a stronger anti microbial activity than the tarragon and ginger oils. Herbal essential oils are candidates to be alternatives in medical applications due to their anti-microbial effects.