6th Clinical Microbiology Conference

Biography

Biography: Ying Shun Zhou

Abstract

Acinetobacter baumannii is an opportunistic pathogen of hospital-acquired infection. The aim of this study was to analysis the 𝛽-lactamases genes prevalence in Acinetobacter baumannii isolated from a hospital in the southwest of China. In this study, 87 A.baumannii strains isolated from a hospital in southern of Sichuan province that were tested for antibiotic susceptibility performed by determination the MIC, molecular genotyping by multilocus sequence typing (MLST), and more importantly, the prevalence of 𝛽-lactamasesgenes (including blaOXA-23, blaOXA-24, blaOXA-58, blaGIM, blaSPM, blaSIM, blaNDM, blaCTX-M, blaSHV and blaTEM) were studied by PCR method. Antibiotic susceptibility test against 13 commonly used antibiotics indicated that most of the stains showed multidrug-resistance. And the result of MLST analysis showed that there were 5 STs in the 87 A. baumannii isolates, and a majority of them was ST195 (69/87). The PCR amplication and sequencing results illustrated that most of these isolates were positive to blaOXA-23 and blaTEM genes, and the prevalence of them were 74.71% and 93.10% respectively. In addition, 1 positive strain for the blaGIM, 1 positive strain for the blaSIM, 1 positive strain for the blaSHV and 2 positive strains for the blaSPM were found. However, all of the isolates were negative to the rest 𝛽-lactamases genes detected including blaOXA-24, blaNDM and blaCTX-M . In conclusion, the 87 A. baumannii isolates in this study showed severe drug resistance, and the main 𝛽 -lactamases genes carried were blaOXA-23gene and blaTEM gene.