Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 3rd International Conference on Medical and Clinical Microbiology Westin Miyako Kyoto, Japan .

Day 2 :

OMICS International Clinical Microbiology 2019 International Conference Keynote Speaker Divocha Valentina  photo
Biography:

Divocha Valentina in 1967 she graduated from I. I. Mechnikov Odessa State University, Faculty of Biology (Department of Virology). In 1973 continued her postgraduate study ate Odessa Institute of Virology and Epidemiology (specialty virology). In 1974 she was awarded her candidate degree with the thesis "Interaction of Coxsackie B viruses with sensitive cell cultures and their antigenic relationships." In 2009 she was awarded her doctoral degree with the thesis entitled "Biological basis antiprotease therapy of influenza."Under her leadership performed a doctoral and two master's theses. Scientific experience is 35 years. I have more than 241 scientific publications, 3 monographs, textbook "Virology" (2012), 12 patents, 4 innovations.
I am currently working as the departament biochemistry Lugansk State Medical University, is the supervisor of the nine research programs in virology and biochemistry.

 

Abstract:

Proteolytic activation is widespread among viruses of different taxonomic groups. Thus, the proteolytic activation of influenza viruses and paramyxoviruses is carried by trypsin-like proteinases of a host cell which hydrolyze the peptide bond between arginine and lysine. Objective: to extract trypsin-like proteinase from the lungs of healthy mice and get  hyperimmune serum to it for the treatment of experimental influenza. We used the lungs of 100 white mice to isolate trypsin–like proteinase; influenza virus A/PR/8/34; white rats were used to produce hyperimmune  antiproteinase sera to study their protective function in the body of white mice infected with a lethal dose of influenza A virus. From the lungs of healthy mice 6 isoforms of trypsin – like proteinases were isolated, and hyperimmune antiproteinase rats’ sera were obtained to them. At the treatment by these sera the white mice, previously infected with a lethal dose of influenza A virus, 60% of the animals under experiment survived only by the use of the serum to the third isoform of trypsin-like proteinase. In the control group, where no treatment was performed, the infected with a lethal dose of influenza virus animals had a 100% lethality on the 4-5th day after infection. The results obtained show that it is possible to get antiproteinase vaccine for the flu, which will block the flu virus in the intercellular space and disrupt the development of the pathological process.