Day 1 :
National Center for Disease Control & Public Health, Georgia
Keynote: Study of prevalence of Bartonella infections among patients with fever of unknown origin in the country of Georgia
Time : 09:30-10:15
L. Malania, She completed her MD in preventive medicine in Tbilisi State University, Tbilisi, Georgia, 2001 and Ph.D. in epidemiology in Tbilisi State Medical University, Tbilisi, Georgia and Postdoctoral Fellowship of Fulbright. Scholar program at Centers for Disease Control and Prevention, Fort Collins, CO, USA, 2010
Statement of the Problem: Only few years ago the diseases caused by Bartonella spp. were viewed as medical curiosities. During recent years it has become apparent that Bartonella species are associated with many illnesses other than the previously recognized cat-scratch disease, trench fever, Carrion’s disease, neuroretinitis, Peliosis hepatis and bacillary angiomatosis. Bartonella-associated illnesses involve a broad spectrum of signs and symptoms. In the present study, our objective was to detect a presence and diversity of Bartonella species among patients with fever of unknown etiology in Georgia. Methodology & Theoretical Orientation: 298 clinical samples (149 - whole blood, 149 - sera) collected in 2015-2016 from the cases with fever of unknown etiology from different clinics of Georgia had been collected; whole blood were plated out on the Chocolate agar and incubated in anaerobic conditions with 5% CO2 for up to 45 days; DNA was extracted from each blood samples and PCR were run to identify presence of Bartonella spp; have been conducted serological investigation of all 149 clinical (sera) samples. Were used four different antigens by Indirect Fluorescent Assay – B. elizabetae, B. tribocorum, B. quintanae, B. hensalae. Findings: We have screened 149 human blood samples by culturing. No Bartonella isolates were obtained from any of these specimens tested. Out of 149 blood tested by conventional PCR assay using gltA primers, only 3 of them gave positive results. Serological investigation of 149 sera 9 samples were confirmed as positive for antibody against B. hensalae and 16 samples were positive on B. quintanae antigen. Totally 16,77% of enrolled cases were positive. Conclusion & Significance: As a result of this study, data on the distribution of Bartonella bacteria in humans are collected for the first time in Georgia. Next steps will be to determine factors associated with the emergence of bartonellosis in Georgia.
Universiti Malaysia Terengganu, Malaysia
Time : 10:15-11:00
Fatimah Binti Hashim has her expertise in protozoology and cytotoxicity based on microscopy analyses. Most of the works are on cytotoxic effects of plant compound, plant extract, lanthanide complexes on Acanthamoeba sp. (corneal scrapping and environmental isolates). Recent works are on isolation and identification of protozoan species from fish gills. Microscopy analysis based on fluorescence, scanning and electron microscopy are her interest techniques for cytotoxicity and protozoan morphological identification. Other interest are on molecular work of genotoxicity specifically by alkaline comet assay technique and DNA laddering assay.
The activity of lanthanide with different chelating agents in protozoan species, have not been reported so far. In this study, antiamoebicity of lanthanide complexes with different chelating agents (as ligands) were assessed on a clinical isolate of Acanthamoeba sp. Complex of lanthanide elements such as europium, cerium, prasedomium, nadomium, dysprodium and samarium salt with EO3, EO4, EO5 as well as 18C6, as ligands were compared for their cytotoxicity effect on Acanthamoeba. We also found that cytotoxicity of different ligand on lanthanide might induce different type of cell death which are apoptosis, autophagic and necrosis cell death. Computational model of different ligands of Samarium (Sm) with EO5 or 18C6 interaction with profilin 1B (PDB ID: 1ACF), an actin-binding protein in Acanthamoeba cells showed possible interaction for the SmEO5 complex with profilin 1B, however, the Sm with 18C6 ligand model was unable to identify possible docking site at potential on profilin. This lack of interaction is due to tertiary conformation of profilin does not favour the generation of hydrogen bond with the Sm18C6 cyclic structure. For the SmEO5, the protein-ligand predicted interaction is at hydrophilic pockets of the protein located at Thr and Ser residues. We also found that level of cytoxicity of lanthanides to Acanthamoeba is inflected by their position in the periodic table. Across the periodic table from left to right, the reactivity and relative atomic mass of lanthanides element increases. Our assessment showed that the toxicity of lanthanides decreases as the atomic number increases.